Hotspot

rps16-trnQ-UUG

LSC · 6388-7859 · 1472 bp

Back to Fallopia

Pi 0.059

MarkerSeek score 71.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1472
variable_sites: 105
pi: 0.059
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGGCAGCAACATACCCTTTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1499-1949 bp	1.0	89.5
rps16-trnQ-UUG_p2	TGGCAGCAACATACCCTTTT	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	2065-2518 bp	1.0	88.9
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.1	57.9	1659-2114 bp	0.75	77.3
rps16-trnQ-UUG_p4	TGGCAGCAACATACCCTTTT	GATTTTGGCGGGAATTGACG	45.0	50.0	58.0	58.1	2067 bp	0.25	49.2
rps16-trnQ-UUG_p5	TGGCAGCAACATACCCTTTT	GCGGGAATTGACGAACAATC	45.0	50.0	58.0	57.8	2060 bp	0.25	48.5