Hotspot

ycf3

LSC · 45453-47444 · 1992 bp

Back to Fagus

Pi 0.00155
MarkerSeek score 53.00
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1992
variable_sites
12
pi
0.00155
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 TTGGTTCTTTCCGGAGAACC ACAATTCCTTTGTTTGTGGGA 50.0 38.1 57.7 56.2 2180-2185 bp 1.0 55.8
ycf3_p2 TTGGTTCTTTCCGGAGAACC TTCCTTTGTTTGTGGGATCA 50.0 40.0 57.7 55.0 2176-2181 bp 1.0 54.9
ycf3_p3 TTGGTTCTTTCCGGAGAACC TCCTTTGTTTGTGGGATCAT 50.0 40.0 57.7 54.8 2175-2180 bp 1.0 52.9
ycf3_p4 TTGGTTCTTTCCGGAGAACC CAATTCCTTTGTTTGTGGGATCA 50.0 39.1 57.7 57.8 2179-2184 bp 1.0 52.8
ycf3_p5 TTGGTTCTTTCCGGAGAACC ACAATTCCTTTGTTTGTGGGATC 50.0 39.1 57.7 57.8 2180-2185 bp 1.0 52.8