Hotspot

psaC

SSC · 121184-121429 · 246 bp

Pi 0.00207

MarkerSeek score 21.00

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	TCCTGTGAGCAAAATAGGGC	TTGGACCGGCTATTGTTTCC	50.0	50.0	57.9	58.2	1004-1008 bp	1.0	80.5
psaC_p2	TCCTGTGAGCAAAATAGGGC	CCGCATGAATCGATACGGAA	50.0	50.0	57.9	58.2	806-810 bp	1.0	80.5
psaC_p3	CTGTGAGCAAAATAGGGCCT	TTGGACCGGCTATTGTTTCC	50.0	50.0	57.9	58.2	1002-1006 bp	1.0	80.4
psaC_p4	CTGTGAGCAAAATAGGGCCT	CCGCATGAATCGATACGGAA	50.0	50.0	57.9	58.2	804-808 bp	1.0	80.4
psaC_p5	TCCTGTGAGCAAAATAGGGC	ATCAAAGCATCTTGGCCCTC	50.0	50.0	57.9	58.2	826-830 bp	1.0	80.3