Hotspot

ccsA

SSC · 118260-119216 · 957 bp

Back to Fagus

Pi 0.00357
MarkerSeek score 46.80
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
957
variable_sites
14
pi
0.00357
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GCGGCATGGCATCTTATCAA AGGTTCGTGATGGCTTACAC 50.0 50.0 59.0 57.9 1125 bp 1.0 72.1
ccsA_p2 GCGGCATGGCATCTTATCAA TGGCTTACACGACGTTTTCT 50.0 45.0 59.0 57.8 1115 bp 1.0 71.0
ccsA_p3 GCGGCATGGCATCTTATCAA TCAAAAGGTTCGTGATGGCT 50.0 45.0 59.0 57.7 1130 bp 1.0 70.7
ccsA_p4 CGGCATGGCATCTTATCAAAG AGGTTCGTGATGGCTTACAC 47.6 50.0 57.7 57.9 1124 bp 1.0 70.2
ccsA_p5 GCGGCATGGCATCTTATCAA TGATGGCTTACACGACGTTT 50.0 45.0 59.0 57.8 1118 bp 0.909091 67.9