Hotspot

rpl32-trnL-UAG

SSC · 116923-118094 · 1172 bp

Back to Fagus

Pi 0.0045
MarkerSeek score 53.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1172
variable_sites
20
pi
0.0045
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCGAAATCTCTTTCTACAGGGA AGCGTGTCTACCGATTTCAC 45.5 50.0 57.9 58.0 1220-1257 bp 1.0 66.7
rpl32-trnL-UAG_p2 GCGAAATCTCTTTCTACAGGGA CAGCGTGTCTACCGATTTCA 45.5 50.0 57.9 58.0 1221-1258 bp 1.0 66.7
rpl32-trnL-UAG_p3 AGCGAAATCTCTTTCTACAGGG AGCGTGTCTACCGATTTCAC 45.5 50.0 57.9 58.0 1221-1258 bp 1.0 66.7
rpl32-trnL-UAG_p4 AGCGAAATCTCTTTCTACAGGG CAGCGTGTCTACCGATTTCA 45.5 50.0 57.9 58.0 1222-1259 bp 1.0 66.6
rpl32-trnL-UAG_p5 TGAAAGCTTTTTCGTTAGCGA AGCGTGTCTACCGATTTCAC 38.1 50.0 56.8 58.0 1237-1274 bp 1.0 65.8