Hotspot

trnS-UGA-psbZ

LSC · 38300-38664 · 365 bp

Back to Fagus

Pi 0.00324
MarkerSeek score 39.00
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
365
variable_sites
2
pi
0.00324
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAAGACCGGAGCTATCAACC CGACCAACCATCAGGAGAAG 55.0 55.0 58.1 58.0 501-509 bp 1.0 80.2
trnS-UGA-psbZ_p2 CAAGACCGGAGCTATCAACC CCAACCATCAGGAGAAGCAA 55.0 50.0 58.1 57.8 498-506 bp 1.0 78.7
trnS-UGA-psbZ_p3 CAAGACCGGAGCTATCAACC GACCAACCATCAGGAGAAGC 55.0 55.0 58.1 58.3 500-508 bp 1.0 78.3
trnS-UGA-psbZ_p4 ACAAATCTATTCGCCGAGCA CGACCAACCATCAGGAGAAG 45.0 55.0 57.7 58.0 583-591 bp 1.0 78.2
trnS-UGA-psbZ_p5 CAAGACCGGAGCTATCAACC TCGACCAACCATCAGGAGAA 55.0 50.0 58.1 58.4 502-510 bp 1.0 77.4