Hotspot

trnM-CAU-psbD

LSC · 33841-35531 · 1691 bp

Back to Fagus

Pi 0.00742
MarkerSeek score 56.30
Primer available yes
Label trnM-CAU-psbD

Feature Metadata

label_name
trnM-CAU-psbD
feature_type
igs
region
LSC
length_bp
1691
variable_sites
55
pi
0.00742
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.95
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-psbD_p1 AGTAATGCGCCATGGTAAGG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.0 58.2 1831 bp 0.090909 41.4
trnM-CAU-psbD_p2 AGTAATGCGCCATGGTAAGG GACCGGACCAACCTACAAAA 50.0 50.0 58.0 57.7 1845 bp 0.090909 41.3
trnM-CAU-psbD_p3 AGTAATGCGCCATGGTAAGG TAGACCGGACCAACCTACAA 50.0 50.0 58.0 57.7 1847 bp 0.090909 40.9
trnM-CAU-psbD_p4 AGTAATGCGCCATGGTAAGG GAGCAATAGACCGGACCAAC 50.0 55.0 58.0 58.3 1853 bp 0.090909 40.6
trnM-CAU-psbD_p5 AGTAATGCGCCATGGTAAGG CAAAAACGAAACGGTCCCTC 50.0 50.0 58.0 57.6 1830 bp 0.090909 40.2