Hotspot

rpoB-trnC-GCA

LSC · 27438-28698 · 1261 bp

Back to Fagus

Pi 0.00296
MarkerSeek score 53.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1261
variable_sites
15
pi
0.00296
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ACATTCCCGCATTTCTACCC TTAGGCGACACCCAGATTTG 50.0 50.0 57.9 57.9 1354-1370 bp 1.0 80.2
rpoB-trnC-GCA_p2 ACATTCCCGCATTTCTACCC TTGTTGATTAGGCGACACCC 50.0 50.0 57.9 58.2 1361-1377 bp 1.0 79.6
rpoB-trnC-GCA_p3 GCATTTCTACCCCCAAGCAT TTAGGCGACACCCAGATTTG 50.0 50.0 58.2 57.9 1346-1362 bp 1.0 78.9
rpoB-trnC-GCA_p4 GCATTTCTACCCCCAAGCAT TTGTTGATTAGGCGACACCC 50.0 50.0 58.2 58.2 1353-1369 bp 1.0 78.2
rpoB-trnC-GCA_p5 ACATTCCCGCATTTCTACCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 57.9 58.4 1325-1341 bp 1.0 78.2