Hotspot

atpH-atpI

LSC · 13972-15094 · 1123 bp

Pi 0.00266

MarkerSeek score 61.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 13
pi: 0.00266
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1004-1214 bp	1.0	83.5
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	1089-1299 bp	1.0	83.4
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	974-1184 bp	1.0	83.2
atpH-atpI_p4	GATCCCTTCTACAGCTTGGC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.0	57.8	1059-1269 bp	1.0	83.1
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1005-1215 bp	1.0	82.3