Hotspot

trnS-GCU-trnG-GCC

LSC · 7995-8934 · 940 bp

Back to Fagus

Pi 0.00592
MarkerSeek score 55.40
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
940
variable_sites
18
pi
0.00592
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1007-1011 bp 1.0 53.3
trnS-GCU-trnG-GCC_p2 ACAAAAGCGGAAAGAGAGGG ACGAATCACACTTTTACCACT 50.0 38.1 58.1 55.5 1068-1072 bp 1.0 52.4
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 1008-1009 bp 0.909091 49.4
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 1011-1012 bp 0.909091 48.3
trnS-GCU-trnG-GCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 1010-1011 bp 0.909091 48.3