Hotspot

trnK-UUU-trnQ-UUG

LSC · 4427-6558 · 2132 bp

Back to Fagus

Pi 0.00502
MarkerSeek score 60.00
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
2132
variable_sites
46
pi
0.00502
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 GCGGTCTTACAAGCTCTACC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 2263-2280 bp 1.0 81.1
trnK-UUU-trnQ-UUG_p2 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 2184-2201 bp 1.0 80.5
trnK-UUU-trnQ-UUG_p3 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 2183-2200 bp 1.0 80.5
trnK-UUU-trnQ-UUG_p4 CGGTCTTACAAGCTCTACCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.8 58.1 2262-2279 bp 1.0 80.2
trnK-UUU-trnQ-UUG_p5 ATCAGTCGCGGTCTTACAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 2270-2287 bp 1.0 79.5