Hotspot

trnH-GUG-psbA

LSC · 100-495 · 396 bp

Back to Fagus

Pi 0.00955
MarkerSeek score 54.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
396
variable_sites
11
pi
0.00955
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 514-520 bp 1.0 78.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 528-534 bp 1.0 78.4
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 527-533 bp 1.0 78.3
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 529-535 bp 1.0 78.3
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 55.0 50.0 58.1 57.6 515-521 bp 1.0 78.0