Hotspot

accD

LSC · 57711-59099 · 1389 bp

Back to Fagopyrum

Pi 0.0403

MarkerSeek score 71.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1389
variable_sites: 104
pi: 0.0403
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GCTTGCGAAGTATGGAAGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2642-2722 bp	1.0	89.3
accD_p2	CTGTACTACAGTTCGGTGGC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	2821-2901 bp	1.0	89.0
accD_p3	GCTTGCGAAGTATGGAAGGA	ACCATCCGGCCTAAACAATC	50.0	50.0	58.0	57.9	2693-2773 bp	1.0	89.0
accD_p4	CTGTACTACAGTTCGGTGGC	ACCATCCGGCCTAAACAATC	55.0	50.0	58.0	57.9	2872-2952 bp	1.0	88.7
accD_p5	GCCTGTGTACAAGCTCGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	2738-2818 bp	1.0	88.7