Hotspot

ndhF-rpl32

SSC · 117429-118373 · 945 bp

Back to Fagopyrum

Pi 0.0898
MarkerSeek score 83.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
945
variable_sites
166
pi
0.0898
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AACCCAAAATCCCCTACACG CTTTTAGCATTCCCGGTCGA 50.0 50.0 57.8 58.0 1597-1658 bp 0.666667 74.4
ndhF-rpl32_p2 AACCCAAAATCCCCTACACG TTAGCATTCCCGGTCGAAAG 50.0 50.0 57.8 58.0 1594-1655 bp 0.666667 74.4
ndhF-rpl32_p3 CTAGGAAAAGCCCACATCCG TTAGCATTCCCGGTCGAAAG 55.0 50.0 58.3 58.0 1206-1220 bp 0.5 62.4
ndhF-rpl32_p4 TGTTGTCGTCGGAAAAAGGA CTTTTAGCATTCCCGGTCGA 45.0 50.0 57.7 58.0 1178-1191 bp 0.333333 51.4
ndhF-rpl32_p5 TGTTGTCGTCGGAAAAAGGA TTAGCATTCCCGGTCGAAAG 45.0 50.0 57.7 58.0 1175-1188 bp 0.333333 51.4