Hotspot

ycf4-cemA

LSC · 60828-61633 · 806 bp

Back to Fagopyrum

Pi 0.066

MarkerSeek score 82.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 806
variable_sites: 104
pi: 0.066
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGGGGTCTCGAAAAACAAGT	GTTAGAAAGCTCCACCAGGG	50.0	55.0	58.0	57.9	1305-1398 bp	1.0	90.1
ycf4-cemA_p2	GTCGCATCTTCCTCCGATTT	AGATAGAGCCAACCACCAGT	50.0	50.0	58.1	58.0	1536-1553 bp	0.5	63.1
ycf4-cemA_p3	GGGGGTCTCGAAAAACAAGT	AGATAGAGCCAACCACCAGT	50.0	50.0	58.0	58.0	1836-1853 bp	0.5	62.8
ycf4-cemA_p4	TATGGGATCGCAGGACTCTT	AGATAGAGCCAACCACCAGT	50.0	50.0	57.9	58.0	1672-1689 bp	0.5	62.5
ycf4-cemA_p5	TTCTATGGGATCGCAGGACT	AGATAGAGCCAACCACCAGT	50.0	50.0	57.9	58.0	1675-1692 bp	0.5	62.5