Hotspot

ycf3-trnS-GGA

LSC · 44425-45260 · 836 bp

Back to Fagopyrum

Pi 0.0714

MarkerSeek score 80.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 110
pi: 0.0714
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCCATTTATACGTGATCTAGGCA	TTCCAATGCTACGCCTTGAA	39.1	45.0	56.9	57.8	896-916 bp	1.0	58.0
ycf3-trnS-GGA_p2	TCCATTTATACGTGATCTAGGCA	AGTTCCAATGCTACGCCTTG	39.1	50.0	56.9	58.5	898-918 bp	1.0	55.2
ycf3-trnS-GGA_p3	TCCATTTATACGTGATCTAGGCA	CAGTTCCAATGCTACGCCTT	39.1	50.0	56.9	58.5	899-919 bp	1.0	55.2
ycf3-trnS-GGA_p4	TTCCATTTATACGTGATCTAGGCA	TTCCAATGCTACGCCTTGAA	37.5	45.0	57.6	57.8	897-917 bp	1.0	55.1
ycf3-trnS-GGA_p5	TCCATTTATACGTGATCTAGGCA	TCCAATGCTACGCCTTGAAC	39.1	50.0	56.9	58.6	895-915 bp	1.0	55.1