Hotspot

trnT-GGU-psbD

LSC · 31459-32717 · 1259 bp

Back to Fagopyrum

Pi 0.0669

MarkerSeek score 82.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1259
variable_sites: 161
pi: 0.0669
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	CGCTTTGAGGTTATGAGCCT	AAACTAGGCGCAAAGAACCA	50.0	45.0	58.0	58.0	2383 bp	0.166667	46.2
trnT-GGU-psbD_p2	CGCTTTGAGGTTATGAGCCT	AACTAGGCGCAAAGAACCAA	50.0	45.0	58.0	58.0	2382 bp	0.166667	46.2
trnT-GGU-psbD_p3	CGCTTTGAGGTTATGAGCCT	ACCAAAACTAGGCGCAAAGA	50.0	45.0	58.0	58.0	2387 bp	0.166667	46.2
trnT-GGU-psbD_p4	CGCTTTGAGGTTATGAGCCT	TCAGGGCCCCATAGTAACAA	50.0	50.0	58.0	58.0	2152 bp	0.166667	46.2
trnT-GGU-psbD_p5	CGCTTTGAGGTTATGAGCCT	TTCAGGGCCCCATAGTAACA	50.0	50.0	58.0	58.0	2153 bp	0.166667	46.2