Hotspot

psbM-trnD-GUC

LSC · 29235-30110 · 876 bp

Back to Fagopyrum

Pi 0.065

MarkerSeek score 87.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 102
pi: 0.065
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	CGTTTTGGCTGACCGTTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.1	58.5	1040-1153 bp	1.0	85.0
psbM-trnD-GUC_p2	CGTTTTGGCTGACCGTTTTT	GTTCAATTGGTCAGAGCACC	45.0	50.0	58.1	57.0	1041-1154 bp	1.0	80.9
psbM-trnD-GUC_p3	CGTTTTGGCTGACCGTTTTT	TCAATTGGTCAGAGCACCG	45.0	52.6	58.1	57.8	1039-1152 bp	1.0	79.0
psbM-trnD-GUC_p4	TCGTTTTGGCTGACCGTTTT	TTCAATTGGTCAGAGCACCG	45.0	50.0	58.9	58.5	1041-1154 bp	1.0	78.5
psbM-trnD-GUC_p5	CGTTTTGGCTGACCGTTTTTA	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.9	58.5	1040-1153 bp	1.0	77.2