Hotspot

rpoB-trnC-GCA

LSC · 26032-27310 · 1279 bp

Back to Fagopyrum

Pi 0.0792

MarkerSeek score 84.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1279
variable_sites: 209
pi: 0.0792
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	CTTACCGCTCGGCCATGT	50.0	61.1	57.3	59.8	1247-1327 bp	1.0	56.2
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCG	TTACCGCTCGGCCATGTC	50.0	61.1	57.3	59.8	1246-1326 bp	1.0	56.2
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCG	CTTACCGCTCGGCCATGTC	50.0	63.2	57.3	60.9	1247-1327 bp	1.0	55.6
rpoB-trnC-GCA_p4	ACATTCCCTCATTTCCATCCC	CTTACCGCTCGGCCATGT	47.6	61.1	57.7	59.8	1249-1329 bp	1.0	52.0
rpoB-trnC-GCA_p5	ACATTCCCTCATTTCCATCCC	TTACCGCTCGGCCATGTC	47.6	61.1	57.7	59.8	1248-1328 bp	1.0	52.0