Hotspot

atpH-atpI

LSC · 13131-13789 · 659 bp

Back to Fagopyrum

Pi 0.0811

MarkerSeek score 77.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 659
variable_sites: 93
pi: 0.0811
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TTCTGGCTGTCTCGCAATAC	CCCTTGGGTTCCCTTTATCG	50.0	55.0	58.0	57.9	1252-1261 bp	1.0	88.3
atpH-atpI_p2	TTCTGGCTGTCTCGCAATAC	CCTTGGGTTCCCTTTATCGG	50.0	55.0	58.0	57.9	1251-1260 bp	1.0	88.2
atpH-atpI_p3	TTCTGGCTGTCTCGCAATAC	CTTGGGTTCCCTTTATCGGG	50.0	55.0	58.0	57.9	1250-1259 bp	1.0	88.2
atpH-atpI_p4	TTCTGGCTGTCTCGCAATAC	CCACAAACCATTCCGACTGA	50.0	50.0	58.0	58.1	1347-1356 bp	1.0	87.4
atpH-atpI_p5	TTCTGGCTGTCTCGCAATAC	CTTATTAAGCTCAGCCGCCA	50.0	50.0	58.0	58.0	1381-1389 bp	0.833333	81.0