Hotspot

trnS-GCU-trnG-GCC

LSC · 7614-8424 · 811 bp

Back to Fagopyrum

Pi 0.0998

MarkerSeek score 83.80

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 146
pi: 0.0998
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	TTTCCGTGAAAGACCCTGTG	TTCATCGGGAGGGAAAGGAT	50.0	50.0	58.0	58.1	1429-1584 bp	0.5	64.1
trnS-GCU-trnG-GCC_p2	TTTCCGTGAAAGACCCTGTG	TCATCGGGAGGGAAAGGATT	50.0	50.0	58.0	58.1	1428-1583 bp	0.5	64.0
trnS-GCU-trnG-GCC_p3	CCCTGTGAGGTTTTCCACAA	TCATCGGGAGGGAAAGGATT	50.0	50.0	57.9	58.1	1415-1570 bp	0.5	63.9
trnS-GCU-trnG-GCC_p4	GTTTGGCAAGCTGCTGTAAG	TTCATCGGGAGGGAAAGGAT	50.0	50.0	57.9	58.1	1569-1724 bp	0.5	63.6
trnS-GCU-trnG-GCC_p5	GTTTGGCAAGCTGCTGTAAG	TCATCGGGAGGGAAAGGATT	50.0	50.0	57.9	58.1	1568-1723 bp	0.5	63.5