Hotspot

ndhG

SSC · 121130-121666 · 537 bp

Back to Fagaropsis

Pi 0.00186

MarkerSeek score 44.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 537
variable_sites: 1
pi: 0.00186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AGTTCAAGACACATCAGGGC	ACGACCGTCACGAATTGAAT	50.0	45.0	57.8	57.9	1387-1397 bp	1.0	78.8
ndhG_p2	AGTTCAAGACACATCAGGGC	ACTGTATCCGCACCAATTCC	50.0	50.0	57.8	58.0	1037 bp	1.0	78.6
ndhG_p3	AGTTCAAGACACATCAGGGC	AAGTATGCGTTCGCGTATGT	50.0	45.0	57.8	58.1	1573-1583 bp	1.0	78.6
ndhG_p4	AGTTCAAGACACATCAGGGC	CTTGTGAAGTATGCGTTCGC	50.0	50.0	57.8	57.9	1579-1589 bp	1.0	78.1
ndhG_p5	AGTTCAAGACACATCAGGGC	GCTTGTGAAGTATGCGTTCG	50.0	50.0	57.8	57.9	1580-1590 bp	1.0	78.1