Hotspot

trnL-CAA-ycf2

IRa · 147585-148596 · 1012 bp

Back to Fagaropsis

Pi 0.00395

MarkerSeek score 61.00

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 1012
variable_sites: 4
pi: 0.00395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	AGATGGCTTTTTCCGGATGA	50.0	45.0	58.0	57.2	1123 bp	1.0	73.8
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	AGATGGCTTTTTCCGGATGA	50.0	45.0	58.0	57.2	1125 bp	1.0	73.8
trnL-CAA-ycf2_p3	GCCTTGGTGGTGAAATGGTA	AGATGGCTTTTTCCGGATGA	50.0	45.0	58.1	57.2	1135 bp	1.0	73.2
trnL-CAA-ycf2_p4	TGGTGAAATGGTAGACACGC	AGATGGCTTTTTCCGGATGA	50.0	45.0	58.2	57.2	1128 bp	1.0	72.3
trnL-CAA-ycf2_p5	GAAATGGTAGACACGCGAGA	AGATGGCTTTTTCCGGATGA	50.0	45.0	57.7	57.2	1124 bp	1.0	71.6