Hotspot

ndhF-trnL-UAG

SSC · 115070-116978 · 1909 bp

Back to Fagaropsis

Pi 0.00367

MarkerSeek score 54.10

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1909
variable_sites: 7
pi: 0.00367
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	GCGAAGATGTTTTGTTGCGA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	2049-2061 bp	1.0	80.0
ndhF-trnL-UAG_p2	GCGAAGATGTTTTGTTGCGA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	2050-2062 bp	1.0	80.0
ndhF-trnL-UAG_p3	GCGAAGATGTTTTGTTGCGA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	2059-2071 bp	1.0	79.1
ndhF-trnL-UAG_p4	GCGAAGATGTTTTGTTGCGA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	58.0	57.8	2058-2070 bp	1.0	78.5
ndhF-trnL-UAG_p5	GCGAAGATGTTTTGTTGCGA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	58.0	57.8	2057-2069 bp	1.0	78.5