Hotspot

ycf2-trnL-CAA

IRb · 95436-96447 · 1012 bp

Back to Fagaropsis

Pi 0.00395

MarkerSeek score 61.00

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 1012
variable_sites: 4
pi: 0.00395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	AGATGGCTTTTTCCGGATGA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.2	58.0	1123 bp	1.0	73.8
ycf2-trnL-CAA_p2	AGATGGCTTTTTCCGGATGA	TGAAATGGTAGACACGCGAG	45.0	50.0	57.2	58.0	1125 bp	1.0	73.8
ycf2-trnL-CAA_p3	AGATGGCTTTTTCCGGATGA	GCCTTGGTGGTGAAATGGTA	45.0	50.0	57.2	58.1	1135 bp	1.0	73.2
ycf2-trnL-CAA_p4	AGATGGCTTTTTCCGGATGA	TGGTGAAATGGTAGACACGC	45.0	50.0	57.2	58.2	1128 bp	1.0	72.3
ycf2-trnL-CAA_p5	AGATGGCTTTTTCCGGATGA	GAAATGGTAGACACGCGAGA	45.0	50.0	57.2	57.7	1124 bp	1.0	71.6