Hotspot

psbT-psbN

LSC · 76394-76468 · 75 bp

Back to Fagaropsis

Pi 0.0267

MarkerSeek score 67.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 2
pi: 0.0267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	50.0	55.0	57.6	58.4	136 bp	1.0	74.9
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	GATCCATTCGAGGAACACGG	50.0	55.0	57.5	58.4	134 bp	1.0	74.0
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	GGCAACCCTCTCAACAACTA	50.0	50.0	57.6	57.4	159 bp	1.0	73.6
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	AGATCCATTCGAGGAACACG	50.0	50.0	57.6	57.4	137 bp	1.0	73.6
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	50.0	50.0	57.5	57.4	157 bp	1.0	72.8