Hotspot

psbE-petL

LSC · 66520-67864 · 1345 bp

Back to Fagaropsis

Pi 0.00225

MarkerSeek score 61.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1345
variable_sites: 3
pi: 0.00225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	ACCAAGAAATAGAGCCGAGG	50.0	50.0	57.9	57.0	1521-1536 bp	1.0	72.6
psbE-petL_p2	CAACCCGCAATGAATAGGGA	CCAAGAAATAGAGCCGAGGT	50.0	50.0	57.9	57.0	1520-1535 bp	1.0	72.6
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	ACCAAGAAATAGAGCCGAGG	50.0	50.0	57.8	57.0	1444-1459 bp	1.0	71.8
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	CCAAGAAATAGAGCCGAGGT	50.0	50.0	57.8	57.0	1443-1458 bp	1.0	71.8
psbE-petL_p5	ACGAATAACCAACCCGCAAT	CCAAGAAATAGAGCCGAGGT	45.0	50.0	58.2	57.0	1529-1544 bp	1.0	71.6