Hotspot

accD-psaI

LSC · 60437-61152 · 716 bp

Back to Fagaropsis

Pi 0.00559

MarkerSeek score 62.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 4
pi: 0.00559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ATCGTACCACGTAACCCTCT	AAAGAAGCCATTGCCATTGC	50.0	45.0	57.9	57.9	877-878 bp	1.0	78.5
accD-psaI_p2	ATCGTACCACGTAACCCTCT	ATTGCCATTGCCGGAAAAAC	50.0	45.0	57.9	58.2	868-869 bp	1.0	77.7
accD-psaI_p3	ATCGTACCACGTAACCCTCT	CATTGCCATTGCCGGAAAAA	50.0	45.0	57.9	58.2	869-870 bp	1.0	77.7
accD-psaI_p4	ATCGTACCACGTAACCCTCT	GCCATTGCCGGAAAAACTAG	50.0	50.0	57.9	57.7	865-866 bp	1.0	77.1
accD-psaI_p5	ATCGTACCACGTAACCCTCT	TGCCATTGCCGGAAAAACTA	50.0	45.0	57.9	58.4	866-867 bp	1.0	76.3