Hotspot

petN-psbM

LSC · 29555-30858 · 1304 bp

Back to Fagaropsis

Pi 0.00537

MarkerSeek score 61.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1304
variable_sites: 7
pi: 0.00537
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACATTTTCCCTTTCGCTCGT	TGTTCGTTCTAGTTCCTACTGC	45.0	45.5	58.1	57.8	1417 bp	1.0	62.2
petN-psbM_p2	CTTTCGCTCGTAGTATGGGG	TGTTCGTTCTAGTTCCTACTGC	55.0	45.5	57.9	57.8	1408 bp	1.0	62.0
petN-psbM_p3	CCCTTTCGCTCGTAGTATGG	TGTTCGTTCTAGTTCCTACTGC	55.0	45.5	57.9	57.8	1410 bp	1.0	61.9
petN-psbM_p4	CCTTTCGCTCGTAGTATGGG	TGTTCGTTCTAGTTCCTACTGC	55.0	45.5	57.9	57.8	1409 bp	1.0	61.9
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TGTTCGTTCTAGTTCCTACTGC	50.0	45.5	58.2	57.8	1455 bp	1.0	61.8