Hotspot

psbK-psbI

LSC · 7580-7964 · 385 bp

Back to Fagaropsis

Pi 0.00779

MarkerSeek score 49.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 3
pi: 0.00779
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	CGCCCTGGATCATTAGACAG	50.0	55.0	57.9	57.8	504-505 bp	1.0	78.7
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	CGCCCTGGATCATTAGACAG	45.0	55.0	58.3	57.8	501-502 bp	1.0	76.7
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	CGCCCTGGATCATTAGACAG	45.0	55.0	58.3	57.8	502-503 bp	1.0	76.7
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	CGCCCTGGATCATTAGACAG	45.0	55.0	58.3	57.8	503-504 bp	1.0	76.7
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	ACGCCCTGGATCATTAGACA	50.0	50.0	57.9	58.5	505-506 bp	1.0	76.0