Hotspot

ycf1

SSC · 129524-135046 · 5523 bp

Back to Exochorda

Pi 0.00362

MarkerSeek score 49.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5523
variable_sites: 30
pi: 0.00362
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAGTCTAAGCAGGAGACAA	GCAGGCCAAGCAGATCTTAA	45.0	50.0	55.5	58.2	235 bp	1.0	59.2
ycf1_p2	ATCAGTCTAAGCAGGAGACA	GCAGGCCAAGCAGATCTTAA	45.0	50.0	55.3	58.2	236 bp	1.0	57.3
ycf1_p3	TCAGTCTAAGCAGGAGACAA	GGAAAAAGAATCTGCAGGCC	45.0	50.0	55.5	57.3	248 bp	1.0	55.8
ycf1_p4	TCAGTCTAAGCAGGAGACAA	GGGAAAAAGAATCTGCAGGC	45.0	50.0	55.5	57.3	249 bp	1.0	55.8
ycf1_p5	TCAGTCTAAGCAGGAGACAAT	GCAGGCCAAGCAGATCTTAA	42.9	50.0	56.1	58.2	235 bp	1.0	55.8