Hotspot

clpP

LSC · 74108-76187 · 2080 bp

Back to Exochorda

Pi 0.00192

MarkerSeek score 48.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2080
variable_sites: 6
pi: 0.00192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AATCCCAAATCACGGATTCG	TCACATCAAAGCGGGATACA	45.0	45.0	56.2	56.9	2172-2182 bp	1.0	57.3
clpP_p2	AATCCCAAATCACGGATTCG	ACATCAAAGCGGGATACAGT	45.0	45.0	56.2	56.9	2170-2180 bp	1.0	57.2
clpP_p3	AATCCCAAATCACGGATTCG	TCACATCAAAGCGGGATACAG	45.0	47.6	56.2	58.1	2172-2182 bp	1.0	57.1
clpP_p4	AATCCCAAATCACGGATTCG	GCTTTCACATCAAAGCGGGA	45.0	50.0	56.2	59.1	2176-2186 bp	1.0	56.9
clpP_p5	AAATCCCAAATCACGGATTCG	TCACATCAAAGCGGGATACA	42.9	45.0	56.9	56.9	2173-2183 bp	1.0	55.3