Hotspot

rpl32-trnL-UAG

SSC · 118165-119714 · 1550 bp

Back to Exochorda

Pi 0.00774

MarkerSeek score 56.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1550
variable_sites: 18
pi: 0.00774
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTAAAGGGTATTGGGCAGCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1674-1723 bp	1.0	80.1
rpl32-trnL-UAG_p2	GTAAAGGGTATTGGGCAGCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	1675-1724 bp	1.0	80.1
rpl32-trnL-UAG_p3	AAAGGGTATTGGGCAGCATT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.7	58.0	1672-1721 bp	1.0	79.2
rpl32-trnL-UAG_p4	AAAGGGTATTGGGCAGCATT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.7	58.0	1673-1722 bp	1.0	79.2
rpl32-trnL-UAG_p5	GTAAAGGGTATTGGGCAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1684-1733 bp	1.0	79.2