Hotspot

petL-petG

LSC · 70036-70196 · 161 bp

Back to Exochorda

Pi 0.00414

MarkerSeek score 28.20

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 161
variable_sites: 1
pi: 0.00414
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TTGCGGGATGGGTAAATGTC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.2	58.0	807 bp	1.0	78.7
petL-petG_p2	TTGCGGGATGGGTAAATGTC	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.2	58.0	1018 bp	1.0	78.6
petL-petG_p3	TTGCGGGATGGGTAAATGTC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.2	58.0	785 bp	1.0	78.6
petL-petG_p4	TTGCGGGATGGGTAAATGTC	GGAAGTAGGAGTAGGGGGTC	50.0	60.0	58.2	57.9	641 bp	1.0	78.4
petL-petG_p5	TTGCGGGATGGGTAAATGTC	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.2	57.9	771 bp	1.0	78.2