Hotspot

atpB-rbcL

LSC · 58510-59290 · 781 bp

Back to Exochorda

Pi 0.00427

MarkerSeek score 51.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 781
variable_sites: 5
pi: 0.00427
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CCCCAGGACCAGACGTAG	CACCAGCTTTGAATCCAACAC	66.7	47.6	58.4	58.0	864 bp	1.0	53.3
atpB-rbcL_p2	CCCCAGGACCAGACGTAG	ACCAGCTTTGAATCCAACACT	66.7	42.9	58.4	58.1	863 bp	1.0	53.0
atpB-rbcL_p3	CCCCAGGACCAGACGTAG	ACACCAGCTTTGAATCCAACA	66.7	42.9	58.4	58.3	865 bp	1.0	50.7
atpB-rbcL_p4	CCCCAGGACCAGACGTAG	AGTCTCTGTTTGTGGTGACA	66.7	45.0	58.4	56.6	836 bp	1.0	50.4
atpB-rbcL_p5	CCCCAGGACCAGACGTAG	GCTTTGAATCCAACACTTGCT	66.7	42.9	58.4	57.6	859 bp	1.0	50.4