Hotspot

psaA-ycf3

LSC · 44987-45724 · 738 bp

Back to Exochorda

Pi 0.00452

MarkerSeek score 61.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 5
pi: 0.00452
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	890-898 bp	1.0	76.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAGGCTTGGTTCGATCAAG	50.0	55.0	57.9	58.6	930-938 bp	1.0	74.5
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGAAACAAGCTATAGCGCT	50.0	45.0	57.9	57.3	899-907 bp	1.0	74.0
psaA-ycf3_p4	TTGAGAAATGACCCGGTCTG	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.5	57.6	910-918 bp	1.0	73.8
psaA-ycf3_p5	TTTAACTTCTGGTTCCGGCG	GCTATAGCGCTTACTCCTGG	50.0	55.0	58.5	57.6	843-851 bp	1.0	73.7