Hotspot

trnT-GGU-psbD

LSC · 34374-35884 · 1511 bp

Back to Exochorda

Pi 0.00399

MarkerSeek score 57.70

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1511
variable_sites: 9
pi: 0.00399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	55.0	50.0	58.1	58.0	1660-1679 bp	1.0	81.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.1	58.0	1663-1682 bp	1.0	81.2
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	55.0	50.0	58.1	58.0	1662-1681 bp	1.0	81.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.1	58.0	1665-1684 bp	1.0	81.1
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.3	58.0	1637-1656 bp	1.0	79.7