Hotspot

atpH-atpI

LSC · 14573-15725 · 1153 bp

Back to Exochorda

Pi 0.00289

MarkerSeek score 60.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1153
variable_sites: 5
pi: 0.00289
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	1209-1211 bp	1.0	80.0
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1238-1240 bp	1.0	79.1
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.2	58.0	1210-1212 bp	1.0	78.8
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1239-1241 bp	1.0	78.0
atpH-atpI_p5	AATAGAAGCAAGTCCGACGG	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.7	58.0	1243-1245 bp	1.0	77.9