Hotspot

ycf1

SSC · 129796-135375 · 5580 bp

Back to Exbucklandia

Pi 0.00185

MarkerSeek score 43.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5580
variable_sites: 15
pi: 0.00185
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTGTGTTTTTCCCCATCCA	TAATCCTGATCAAGCCGACG	45.0	50.0	56.9	57.5	243 bp	1.0	68.4
ycf1_p2	TCTGTGTTTTTCCCCATCCAA	TAATCCTGATCAAGCCGACG	42.9	50.0	57.7	57.5	243 bp	1.0	66.4
ycf1_p3	TTCTGTGTTTTTCCCCATCCA	TAATCCTGATCAAGCCGACG	42.9	50.0	57.7	57.5	244 bp	1.0	66.3
ycf1_p4	TCTGTGTTTTTCCCCATCCA	ATAATCCTGATCAAGCCGACG	45.0	47.6	56.9	58.0	244 bp	1.0	64.2
ycf1_p5	TCTGTGTTTTTCCCCATCCA	AGCCGACGAAGTAGCTTTG	45.0	52.6	56.9	57.9	231 bp	1.0	63.6