Hotspot

rpoA

LSC · 82659-83672 · 1014 bp

Back to Exbucklandia

Pi 0.00197

MarkerSeek score 60.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1014
variable_sites: 3
pi: 0.00197
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CGCTTCAAAGTGAATTCTCCC	AATGGCTGTAGACCTCCCAA	47.6	50.0	57.5	58.3	1213 bp	1.0	65.7
rpoA_p2	CGCTTCAAAGTGAATTCTCCC	ATGGCTGTAGACCTCCCAAA	47.6	50.0	57.5	58.3	1212 bp	1.0	65.7
rpoA_p3	CGCTTCAAAGTGAATTCTCCC	TGGCTGTAGACCTCCCAAAA	47.6	50.0	57.5	58.6	1211 bp	1.0	63.9
rpoA_p4	CGCTTCAAAGTGAATTCTCCC	TAATGGCTGTAGACCTCCCA	47.6	50.0	57.5	57.4	1214 bp	1.0	63.9
rpoA_p5	CGCTTCAAAGTGAATTCTCCC	GGCTGTAGACCTCCCAAAAA	47.6	50.0	57.5	57.4	1210 bp	1.0	63.7