Hotspot

rps15-ycf1

SSC · 129421-129795 · 375 bp

Back to Exbucklandia

Pi 0.00711

MarkerSeek score 44.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 375
variable_sites: 4
pi: 0.00711
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TAAGCGAGTAACCGTTGACG	TGGCAGTCGTTTCAGTATGG	50.0	50.0	58.0	57.9	630 bp	1.0	79.6
rps15-ycf1_p2	TAAGCGAGTAACCGTTGACG	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.0	57.9	629 bp	1.0	79.6
rps15-ycf1_p3	ATTTCCTAACTGCTCTGGCG	TGGCAGTCGTTTCAGTATGG	50.0	50.0	58.0	57.9	1086 bp	1.0	79.5
rps15-ycf1_p4	ATTTCCTAACTGCTCTGGCG	GGCAGTCGTTTCAGTATGGT	50.0	50.0	58.0	57.9	1085 bp	1.0	79.4
rps15-ycf1_p5	AACACCATGCATTGATGGGT	TGGCAGTCGTTTCAGTATGG	45.0	50.0	58.1	57.9	888 bp	1.0	79.2