Hotspot

ndhF-rpl32

SSC · 117600-118751 · 1152 bp

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Pi 0.00289

MarkerSeek score 61.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1152
variable_sites: 5
pi: 0.00289
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTCGGAAAAAGGAGAAGT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.8	58.0	1325-1334 bp	1.0	79.5
ndhF-rpl32_p2	TTTGTTGCCGTCGGAAAAAG	TTAACGCTGCCCAATATCCC	45.0	50.0	57.8	58.0	1332-1341 bp	1.0	79.3
ndhF-rpl32_p3	CTGGAAGTGGAACGAAAGGT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1283-1292 bp	1.0	78.8
ndhF-rpl32_p4	CCGTCGGAAAAAGGAGAAGT	GAAAGCTTTTAACGCTGCCC	50.0	50.0	57.8	58.3	1333-1342 bp	1.0	77.3
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	GAAAGCTTTTAACGCTGCCC	45.0	50.0	57.8	58.3	1340-1349 bp	1.0	77.0