Hotspot

ycf15-trnL-CAA

IRb · 98426-99048 · 623 bp

Back to Exbucklandia

Pi 0.00535
MarkerSeek score 62.20
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
623
variable_sites
5
pi
0.00535
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 GGTTGTTGCTATCTGCTCCA AAATGGTAGACACGCGAGAC 50.0 50.0 57.9 58.0 736 bp 1.0 79.6
ycf15-trnL-CAA_p2 TGGTTGTTGCTATCTGCTCC AAATGGTAGACACGCGAGAC 50.0 50.0 57.9 58.0 737 bp 1.0 79.6
ycf15-trnL-CAA_p3 GGTTGTTGCTATCTGCTCCA TGAAATGGTAGACACGCGAG 50.0 50.0 57.9 58.0 738 bp 1.0 79.6
ycf15-trnL-CAA_p4 TGGTTGTTGCTATCTGCTCC TGAAATGGTAGACACGCGAG 50.0 50.0 57.9 58.0 739 bp 1.0 79.6
ycf15-trnL-CAA_p5 GGTTGTTGCTATCTGCTCCA GCCTTGGTGGTGAAATGGTA 50.0 50.0 57.9 58.1 748 bp 1.0 79.0