Hotspot

psbE-petL

LSC · 69394-70682 · 1289 bp

Back to Exbucklandia

Pi 0.00259

MarkerSeek score 60.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 5
pi: 0.00259
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GCCGCTAGTAGAAAACCGAA	45.0	50.0	58.0	58.0	1445-1446 bp	1.0	80.1
psbE-petL_p2	TGACAAATAACCAACCCGCA	AAGCCGCTAGTAGAAAACCG	45.0	50.0	58.0	58.0	1447-1448 bp	1.0	80.1
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1434-1435 bp	1.0	79.9
psbE-petL_p4	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1436-1437 bp	1.0	79.8
psbE-petL_p5	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.8	58.0	1357-1358 bp	1.0	79.0