Hotspot

petA-psbJ

LSC · 67566-68609 · 1044 bp

Back to Exbucklandia

Pi 0.00383

MarkerSeek score 48.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1044
variable_sites: 6
pi: 0.00383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ACTGGTATTCCTGTGATCGG	50.0	50.0	58.0	56.7	1238-1241 bp	1.0	69.8
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CTGGTATTCCTGTGATCGGT	50.0	50.0	58.0	56.7	1237-1240 bp	1.0	69.8
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ACTGGTATTCCTGTGATCGG	50.0	50.0	57.8	56.7	1221-1224 bp	1.0	68.9
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	CTGGTATTCCTGTGATCGGT	50.0	50.0	57.8	56.7	1220-1223 bp	1.0	68.9
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	CTGGTATTCCTGTGATCGGT	50.0	50.0	58.2	56.7	1234-1237 bp	1.0	68.7