Hotspot

accD-psaI

LSC · 63044-63731 · 688 bp

Back to Exbucklandia

Pi 0.00291

MarkerSeek score 60.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 3
pi: 0.00291
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AACTCCACGCTTTCTTTCCT	CGGAACTACTAGGCCCACTA	45.0	55.0	57.4	57.9	789-791 bp	1.0	74.7
accD-psaI_p2	ACTCCACGCTTTCTTTCCTT	CGGAACTACTAGGCCCACTA	45.0	55.0	57.4	57.9	788-790 bp	1.0	74.7
accD-psaI_p3	AACTCCACGCTTTCTTTCCT	TTGCAATTGCCGGAACTACT	45.0	45.0	57.4	58.1	799-801 bp	1.0	74.4
accD-psaI_p4	ACTCCACGCTTTCTTTCCTT	TTGCAATTGCCGGAACTACT	45.0	45.0	57.4	58.1	798-800 bp	1.0	74.4
accD-psaI_p5	ACTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.4	57.9	807-809 bp	1.0	74.3