Hotspot

ndhC-trnV-UAC

LSC · 54578-55718 · 1141 bp

Back to Exbucklandia

Pi 0.00292
MarkerSeek score 60.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1141
variable_sites
5
pi
0.00292
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGTCCTTTACTAATCGGAGC AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.2 57.8 1287-1288 bp 1.0 64.3
ndhC-trnV-UAC_p2 ACTCCGGAAATTAGAAATGCCA AGAAGGTCTACGGTTCGAGT 40.9 50.0 58.0 57.8 1262-1263 bp 1.0 62.5
ndhC-trnV-UAC_p3 GGTCCTTTACTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 47.6 50.0 56.9 57.8 1286-1287 bp 1.0 62.0
ndhC-trnV-UAC_p4 TGGTCCTTTACTAATCGGAGC GAAGGTCTACGGTTCGAGTC 47.6 55.0 57.2 57.5 1286-1287 bp 1.0 61.5
ndhC-trnV-UAC_p5 CTGGTCCTTTACTAATCGGAGC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1288-1289 bp 1.0 60.5