Hotspot

trnT-UGU-trnL-UAA

LSC · 50017-51166 · 1150 bp

Back to Exbucklandia

Pi 0.00464

MarkerSeek score 47.10

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1150
variable_sites: 8
pi: 0.00464
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	TCGGTGACCTTTGGAAAAGG	GTTCCTTTACCAACGCAACG	50.0	50.0	58.0	58.0	1570 bp	1.0	80.2
trnT-UGU-trnL-UAA_p2	TCGGTGACCTTTGGAAAAGG	ACGCAGTCAACTCCATTTGT	50.0	45.0	58.0	58.0	1553 bp	1.0	80.2
trnT-UGU-trnL-UAA_p3	CGAAGCCATACCCAATCGAA	GTTCCTTTACCAACGCAACG	50.0	50.0	58.1	58.0	2136-2137 bp	1.0	79.9
trnT-UGU-trnL-UAA_p4	TTTGGAAAAGGGGGAAGACG	GTTCCTTTACCAACGCAACG	50.0	50.0	58.0	58.0	1561 bp	0.333333	53.0
trnT-UGU-trnL-UAA_p5	TTTGGAAAAGGGGGAAGACG	ACGCAGTCAACTCCATTTGT	50.0	45.0	58.0	58.0	1544 bp	0.333333	53.0