Hotspot

rps4-trnT-UGU

LSC · 49587-49943 · 357 bp

Back to Exbucklandia

Pi 0.00373

MarkerSeek score 47.70

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 2
pi: 0.00373
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	515-516 bp	1.0	76.8
rps4-trnT-UGU_p2	AGATCACTTCCGGCTCTAGG	GGTTCGATTCCGATAGCTGG	55.0	55.0	58.3	58.2	474-475 bp	1.0	76.2
rps4-trnT-UGU_p3	AGATCACTTCCGGCTCTAGG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	483-484 bp	1.0	76.0
rps4-trnT-UGU_p4	AGATCACTTCCGGCTCTAGG	CTGTTATGTGAGCCCGCTTA	55.0	50.0	58.3	57.7	536-537 bp	1.0	75.4
rps4-trnT-UGU_p5	AGATCACTTCCGGCTCTAGG	TGTTATGTGAGCCCGCTTAG	55.0	50.0	58.3	57.7	535-536 bp	1.0	75.4