Hotspot

trnT-GGU-psbD

LSC · 34522-35931 · 1410 bp

Back to Exbucklandia

Pi 0.00426

MarkerSeek score 61.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1410
variable_sites: 9
pi: 0.00426
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	CACAAAAACGAAACGGTCCC	50.0	50.0	57.9	58.2	1575 bp	1.0	78.3
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	CCACAAAAACGAAACGGTCC	50.0	50.0	57.9	58.2	1576 bp	1.0	78.3
trnT-GGU-psbD_p3	GCCCTTTTAACTCAGCGGTA	CCCACAAAAACGAAACGGTC	50.0	50.0	57.9	58.2	1577 bp	1.0	78.3
trnT-GGU-psbD_p4	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1574 bp	1.0	77.7
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	AACCCACAAAAACGAAACGG	50.0	45.0	57.9	57.7	1579 bp	1.0	77.4